TXNDC17基因的进化分析+文献综述
关键词：TXNDC17；生物信息学；进化树；蛋白分析
The Evolution Analysis of TXNDC17 Gene
Abstract: The genetic relationship of nine creatures were analyzed which are Homo sapiens, Sarcophilus harrisii, Pseudopodoces humilis, Danio rerio, Chrysemys picta, Xenopus tropicalis, Python bivittatus, Alligator sinensis and Latimeria chalumnae containing TXNDC17 gene and the protein encoded by TXNDC17Gene in human was analyzed and forecasted from the aspects of protein amino acid composition, physical and chemical properties, the subcellular localization, across the membrane area, signal peptide, secondary structure, tertiary structure and functional domains and so on through bioinformatics methods by softwares, such as NCBI, DNAman Expasy and so on. The results show that TXNDC17 gene in the process of evolution is not very conservative and the genetic similarity of nine creatures is only 15.92%. TXNDC17 gene in the process of evolution is pided into two branch, one are bony fish, amphibians and cavity spines fish and another are mammals (including animal placenta and marsupials), birds, lizards and sea crocodilians. The results analyzing the protein encoded by TXNDC17gene in human show that the protein has 124 amino acids and exists TRP14-like conservative domain structure which belongs to Thiordoxin-like super family and it has different phosphorylation sites and does not exist Transmembrane structure and signal peptide sequence in N-terminal sequence which indicate the protein is located in cytoplasmic matrix and does not belong to the membrane proteins or secreted proteins.
Keywords: TXNDC17; Bioinformatics; The evolutionary tree; Protein analysis
目 录
摘要 1
引言 2
1. 材料与方法 2
1.1 物种的获取 2
1.2 基因进化分析 3
1.3 分析方法与工具 3
2. 结果与分析 4
2.1 基因结构与定位分析 4
2.2 九种生物TXNDC17基因的比较和同源进化树的构建。 6
2.3 人类TXNDC17基因所编码蛋白的生物信息学结果与分析 8
3. 讨论与结论 13
参考文献 14
致谢 16
TXNDC17基因的进化分析引言
硫氧还蛋白域17(TXNDC17)基因是一个蛋白编码基因，用来编码与电子载体活动和二硫化物蛋白还原酶的活动相关的蛋白[1]。硫氧还蛋白是细胞中普遍存在的低分子量蛋白质，为生物体所必需。硫氧还蛋白、硫氧还蛋白还原酶和烟酰胺腺嘌呤二核苷磷酸组成硫氧还蛋白系统，具有调节细胞的氧化还原状态 [2]，抗凋亡及促进细胞增殖等功能[3]。硫氧还蛋白能改变植物自交不亲和性状，具有抗旱、耐热、抗氧化胁迫和对抗逆基因的表达进行调控的功能[4]。