摘要目的  利用四溴联苯醚(2,2′,4,4′-tetrabromodiphenyl ether，BDE-47) 建立小鼠肝脏损伤模型，研究曲克芦丁对BDE-47诱导的小鼠肝脏氧化应激的保护作用。方法  建立BDE-47诱导的小鼠肝脏损伤模型，用曲克芦丁处理，设置正常Control组、BDE-47 组、BDE-47（150mg/kg/day）+Troxerutin （150mg/kg/day）组和Troxerutin组共四组，连续喂养12周后处死。以生化检测的方法检测小鼠肝脏中ALT的含量、ROS含量、4-HNE含量、GPX和SOD酶的活力和GSH含量。结果BDE-47能够引起小鼠肝脏细胞中ALT含量升高、ROS与4-HNE含量增加、GPX和SOD酶的活力降低和小分子抗氧化物GSH含量下降；曲克芦丁能使ALT、ROS、4-HNE、GPX、SOD和GSH恢复到对照组水平。而且单独加入曲克芦丁对于ROS与4-HNE含量，GSH含量，GPX和SOD酶是没有影响。结论  曲克芦丁能够有效地减少由于BDE-47所引起的ROS与4-HNE含量提高与GSH含量的减少，并且对于BDE-47所引起的GPX和SOD酶的活力降低的现象能够有效地改善。69174

本文有图2张，表6个，参考文献24篇。

毕业论文关键词: 曲克芦丁  2,2′,4,4′-四溴联苯醚  氧化应激   小鼠肝脏

Troxerutin attenuates oxidative stress induced by BDE-47 in mouse liver 

Abstract Objective： In this study, we established the mouse model of liver injury to research the protective effects of troxerutin on BDE-47-induced hepatic oxidative stress. Method: 24 mice were pided into 4 groups at random：control group, BDE-47 group, BDE-47 150mg/kg/day + Troxerutin 150mg/kg/day group and Troxerutin group. After 12 weeks of treatment, mice were sacrificed to determine the serum level of ALT, ROS and 4-HNEcontent, GPX and SOD enzyme activities, as well as GSH content in the livers of mice. Results: BDE-47 markedly elevated serum ALT level and hepatic ROS and 4-HNE content, decreased the hepatic activities of SOD and GPX and GSH content in mice. However, troxerutin significantly reduced serum ALT level and hepatic ROS and 4-HNE content, increased the hepatic activities of SOD and GPX and GSH content in BDE-47 treated mice. Conclusion:  Troxerutin could effectively protect mouse fromBDE-47-induced liver injury by attenuating ROS and 4-HNE content, increasing the activities of SOD and GPX and GSH content.

Key Words：troxerutin   BDE-47  oxidative stress   mouse liver

目  录

摘要-Ⅰ

Abstract--Ⅱ

目录-Ⅲ

图清单-Ⅳ

表清单-Ⅳ

变量注释表-Ⅳ

1 绪论5

2 材料与方法6

2.1 材料-6

2.2 实验器材6

2.3 方法6

3 结果与分析8

3.1  BDE-47及曲克芦丁对小鼠体重的影响8

3.2  BDE-47对小鼠肝脏损伤的影响及曲克芦丁的保护作用--9

3.3  BDE-47对小鼠氧化损伤的影响及曲克芦丁的保护作用10

4 讨论13

4.1 ALT与肝脏系数--13

4.2 肝细胞的氧化损伤-13

4.3 抗氧化保护系统-13

5 结论-14

参考文献15

致谢-17

图清单

图序号 图名称 页码

图3-1 H&E染色 11

图3-2 小鼠肝脏ROS的含量 12

表清单

表序号 表名称 页码

表3-1 小鼠体重 10

表3-2 小鼠肝脏系数和ALT 11