昆仑雪菊生物活性物质的提取与评价
关键词：昆仑雪菊；醇提；抗氧化；黄酮
Coreopsis tinctoria，s extraction of bioactive substances and evaluation
Abstract: The experiment of kunlun snow chrysanthemum active substances extraction conditions of flavonoids was optimized, the snow chrysanthemum varieties of medium yellow ketone and detect its antioxidant ability. Snow chrysanthemum rate of flavonoids as index, solvent dosage, solvent concentration, extracting time, extracting temperature and particle size, soaking time, microwave irradiation, ultrasound irradiation pretreatment effects on flavonoid yield. Flavonoids of antioxidant capacity respectively by snow chrysanthemum extract on the DPPH, the clearance rate of • OH free radical to inspect. Experimental results show that when the snow chrysanthemum powder particle size less than or equal to 80 mesh, 45 min, soaking pretreatment with 70% ethanol and solid-liquid ratio for 1:30 extraction and water bath shock 2 h rate of flavonoids as the optimal conditions. Ultrasound and microwave pretreatment can greatly improve the flavonoids extraction yield, but the microwave is faster, more province cost. In addition, the snow chrysanthemum extract on the DPPH, OH, there was a clear effect.
Keywords: kunlun snow chrysanthemum; Alcohol extraction; Antioxidant; Evaluation
目录
1 前言 3
1.1 国内外对黄酮类物质的研究 3
1.2 黄酮的提取方法 4
1.3 黄酮化合物分析方法的研究现状 6
1.4 菊花的研究现状 6
1.4.1 菊花的化学成分 6
1.4.2 菊花的功效 7
1.5 菊花中黄酮的研究现状 8
1.5.1 菊花中黄酮的提取 8
1.5.2 菊花中黄酮的分离纯化方法 8
1.6 有效成分的功效评价 8
1.6.1 DPPH 自由基清除能力 8
1.6.2 对•OH的清除作用 9
1.6.3 对超氧基阴离子清除作 9
1.7 本课题的研究内容 9
2 实验材料与方法 10
2.1 实验原材料 10
2.2 实验主要试剂 10
2.3 主要仪器和设备 10
2.4 试验方法 11
2.4.1 昆仑雪菊活性物质黄酮的提取工艺流程 11
2.4.2 标准曲线的绘制 12
2.4.3 昆仑雪菊黄酮醇提条件优化 12
2.4.4 预处理对昆仑雪菊黄酮提取得率的影响 12
2.4.5 HPLC分析黄酮组分和含量 13
2.4.6 提取液抗氧化活性测定 13
3 .实验结果与分析 15
3.1 昆仑雪菊黄酮醇提条件的优化结果 15
3.1.1 芦丁标准曲线绘制 15
3.1.2 乙醇浓度对黄酮提取率的影响 15
3.1.3 醇提料液比对黄酮提取率的影响 16
3.1.4 醇提温度对黄酮提取率的影响 17