拟南芥3-磷酸甘油脱氢酶（AtGPDH）基因的生物信息学分析
毕业论文关键词：拟南芥； GPDH ； 油脂合成 ； 表达分析 ； 生物信息学
Bioinformatics Analysis of the Gene of 3- Glycerol Dehydrogenase（AtGPDH）from Arabidopsis thaliana
Abstract：Crucifer Arabidopsis Arabidopsis (Arabidopsis thaliana), because of its small plants, has the advantages of simple structure, small genome, short growth period, high propagation coefficient, easy genetic transformation characteristics. Therefore, it has become genetic, cellular, developmental, molecular biology studies of model organisms. It has important function in genetics research.The whole genome sequence of Arabidopsis was completed in December, 2000. This plant Arabidopsis was used to study the function of GPDH gene, GPDH is an important enzyme in the synthesis process of oil, improve oil content has the function, in the glycerol synthesis pathway, the fatty acid synthesis pathway, TAG pathway and resistance to osmotic stress and improving the immunity of plants has an important role, for further biological study on the function of GPDH, using bioinformatics methods on protein physicochemical properties of different species, protein homology and phylogenetic tree analysis, subcellular localization, conserved domain, signal peptide, protein structure, gene expression level were predicted and analyzed, results show that there are certain differences the difference of content of relative molecular mass and various amino acids AtGPDH, homology and phylogenetic analysis showed that recently, Arabidopsis thaliana and Brassica napus highest homology and phylogenetic relationship. The GPDH protein had no transmembrane domain and signal peptide, and the expression of GPDH in different tissues and organs was different, and the expression of the seed was the strongest in the seed.. The results of this study will provide a reference for further studies on the biological function of GPDH in Arabidopsis.
Keywords: Arabidopsis ；GPDH ; Oil synthesis ; expression anaiysis ; bioinformatics
目 录
摘要 4
引言 6
1材料 6
1.1生物信息学数据库与软件 6
2分析方法 7
2.1蛋白质序列的获取 7
2.1.1拟南芥G3PDH基因的蛋白质序列的获得 7
2.1.2拟南芥GPDH蛋白的进化分析 7
2.1.3同源序列的获得 7
2.1.4同源序列的对比及系统发育树分析 7
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