Cd、Pb单一及其复合胁迫对凤眼莲生长及生理生化的影响
毕业论文关键字：Pb2+；Cd2+；复合胁迫；凤眼莲；生长；生理生化
Effects of Cd and Pb and Their Combination on Growth and Physiological and Biochemical Characteristics of Crassipes
Abstract: Using Crassipes as the material,using hydroponic method,we studied the effects of different concentrations of Cd and Pb and Their Combination on Growth and Physiological and Biochemical Characteristics of crassipes. The results showed as follow：Under the single processing and low concentration of Pb2+(150 mg/L) , photosynthetic pigment soluble protein,soluble sugar and MDA content had no significant change (P>0.05), with activities of POD and CAT achieving the maximum ; With the increase of concentration, the contents of photosynthetic pigments, soluble protein and soluble sugar continued to reduce and the content of MDA continued to increase. The activities of enzyme were inhibited by different degrees: The activity of SOD continued to rise;When the concentration was 200mg/L, the activity of SOD achieved the maximum and the activities of POD and CAT started to decrease.Under the stress of Cd2+, the contents of photosynthetic pigments, soluble protein and soluble sugar decreased with the increase of the concentration;It is a significant dose - effect relationship. The content of MDA increased significantly;The activities of antioxidant enzymes rised first and fell soon with the increasing of Cd2+;When the concentration of Cd2+ was 40mg/L,activities of POD and CAT achieved to the maximum,SOD achieving to the most as the concentration of Cd2+ was 60mg/L. Cd2+ had more impact than Pb2+. Crassipes had less ability to defend the pollution of Cd2+.When high concentrations of heavy metal coerced,SOD acted as the main role of protection and the protection of POD and CAT fell relatively The combined effects of Cd2+ and Pb2+ on the plant were greater than their single,and the synergy effects of Cd2+ and Pb2+ were observed.
Key Words：Pb2+；Cd2+；combined stress；Eichhornia crassipes；growth；physiological and biochemical characteristics
目 录
摘 要： 1
Abstract 1
引言 2
1 材料与方法 3
1.1 实验材料 3
1.2 处理方法 3
1.3 测定项目与方法 3
1.4 数据分析 3
2 结果与分析 4
2.1 Cd、Pb 单一及复合胁迫对凤眼莲光和色素的影响 4
2.2 Cd、Pb单一及其复合胁迫对凤眼莲可溶性糖含量的影响 4
2.3 Cd、Pb单一及其复合胁迫对凤眼莲可溶性蛋白含量的影响 5
上一篇：蓖麻SOC1基因生物信息学分析