摘要：NADPH氧化酶 是一种广泛存在于生物界产生ROS的关键酶，最近的研究发现呼吸爆发氧化酶 (Respiratory burst oxidasehomologues，Rboh) 是植物ROS的主要生产者。为探究在重金属胁迫下，TaRboh基因对拟南芥生长的影响，测量了拟南芥中脯氨酸（Pro）含量、丙二醛（MDA）含量、超氧化物气化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）活性的变化[1]，通过这些指标的变化情况来反映TaRboh基因对拟南芥抗性的影响。实验表明在重金属铅胁迫下，除丙二醛（MDA），无论是实验对照组还是处理组，各生理指标含量均为转基因型拟南芥＞野生型拟南芥＞突变体型拟南芥，丙二醛（MDA）含量正好与之相反，表现为突变体型＞野生型＞转基因型。总体来说 ，TaRboh基因提高了拟南芥的抗逆性。42961

毕业论文关键词：TaRboh基因；拟南芥；重金属铅；抗逆性

Response of TaRboh gene to Pb in Arabidopsis thaliana

Abstract:NADPH oxidase is a key enzyme widely exists in organisms to produce ROS，a recent study found that respiratory burst oxidase (Rboh) is a major producer of plant ROS. In order to explore under heavy metal stress, effect of TaRboh gene on the growth of Arabidopsis thaliana, Arabidopsis proline measurement (Pro) content, malondialdehyde (MDA) content, superoxide enzyme gasification (SOD), catalase (CAT) and peroxidase (POD) changes the activity of , the change of these indicators to reflect the effect of TaRboh gene on resistance of Arabidopsis. Experimental results show that in the Pb stress, in addition to C two aldehyde (MDA), both the experimental group and control group. Or in the treatment group, the content of each physiological index of transgenic Arabidopsis > Arabidopsis wild type > mutation type Arabidopsis and malondialdehyde (MDA) content is just the opposite, the mutation type > wild type > transgenic. Overall, gene TaRboh improve the stress tolerance in Arabidopsis thaliana.

Keywords:TaRboh gene; Arabidopsis thaliana; Pb; Stress resistance

目    录

 摘  要 1

引  言 2

1 实验材料与方法 2

1.1实验材料 2

1.1.1 供试植物 2

1.1.2  MS培养基 2

1.1.3 固体培养基材料 2

1.1.4实验器材 3

1.2方法 3

1.2.1 MS培养基的制备 3

1.2.2倒平板 3

1.2.3拟南芥培育 3

1.2.4胁迫处理 3

1.2.5生理指标的测定 4

2 结果与分析 4

2.1  TaRboh基因对铅胁迫下拟南芥中抗氧化酶类活性的影响 4

2.1.1 TaRboh基因对铅胁迫下拟南芥中SOD活性的影响 4

2.1.2  TaRboh基因对铅胁迫下拟南芥中POD活性的影响 5

2.1.3 TaRboh基因对铅胁迫下拟南芥中CAT活性的影响 5

2.2 TaRboh基因对铅胁迫下拟南芥中Pro含量的影响 6

2.3 TaRboh基因对铅胁迫下拟南芥中MDA含量的影响 7

3 讨论与结论 8

参考文献