溶液的酸碱性对生物活性分子在纳米材料界面构型的影响
The influence of the solution’s acidity and alkalinity on bioactive molecule in nano-material interface configuration
Abstract: The Surface Enhanced Raman Scattering (SERS) technique has been widely used in many research fields, such as life science, material science, catalysis, and environment chemistry. Utilization of the SERS technique based on nano-particles for studying the biological molecules can incorporate very effectively both the advantage of SERS spectroscopy and those of nano-particles. It can greatly broaden and go deep into the applications of SERS technique in research field concerning biological molecules. For this purpose, our research work prepares the SERS substrate materials having good stability, for detecting the SERS activity of the dopamine in different nano-material surface. Dopamine, L- nor epinepHrine and 3, 4-dihydroxypHenylacetic acid were selected as the representative molecules for our research. Through the electrochemical method, the ultraviolet spectrum and SERS spectra technology study the effect of the pH values on dopamine in different metal nano-particles. Lay the foundation for the mechanism of combining state and interactions between biological molecules to further research and nanometer material effect of structure and properties and molecular and metal surface. It has very important significance for understanding the relationship between molecular structure and its function.
Keywords：Surface Enhanced Raman Scattering；pH value；metal nanoparicles；Dopamine
目录
1 前言 1
1.1 表面增强拉曼散射（SERS）的介绍 1
1.1.1 拉曼散射的基本原理及特点 1
1.1.2 SERS现象及原理 2
1.2 金属纳米材料及其在SERS光谱中的研究 3
1.2.1 纳米材料简介 3
1.2.2 纳米材料的特性 4
1.2.3 纳米材料的制备方法 5
1.2.4 SERS基底的制备及应用现状 5
1.2.5 SERS光谱的应用研究 6
1.3 电化学研究 7
1.4 多巴胺及类似物简介 8
1.5 课题的目的和意义 9
2 实验部分 9
2.1 试剂和仪器 9
2.1.1 试剂 9
2.1.2 仪器 10
2.2 实验步骤 10
2.2.1试剂的制备 10
2.2.2不同pH值下PBS缓冲溶液的配制 10
2.2.3 纳米银基底的制备 11
2.2.4银纳米探针的制备 11
2.2.5电化学测试 11
2.2.6表面增强拉曼光谱测试 11
3 实验结果和讨论 11
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