摘    要 目的：探讨小胶质细胞激活剂脂多糖对大鼠海马和皮层小胶质细胞激活及其对神经元的影响。方法：SD大鼠腹腔注射脂多糖诱导脑炎症模型，免疫组化染色观察海马DG区和皮层Iba-1阳性细胞变化，并应用HE染色进一步观察海马DG区和皮层神经元改变。结果：免疫组化实验显示，SD大鼠腹腔注射脂多糖7d诱导海马DG区和皮层Iba-1阳性细胞明显增加，胞体明显增大，突起粗短深染，提示小胶质细胞激活。HE染色发现部分脂多糖组大鼠海马DG区神经元可见细胞变形和深染现象。结论：脂多糖诱导大鼠海马DG区和皮层小胶质细胞激活，小胶质细胞激活可能在脑损伤后的脑内炎症反应起一定的作用。90464

毕业论文关键词：脂多糖； 脑损伤； 小胶质细胞； 神经元

Abstract Objectives: To explore the microglial activator the lipopolysaccharide (LPS) induces microglial activation and its effects on neurons in the rat hippocampus and cortex。 Methods: Brain injury induced by intraperitoneal injection of LPS (2。5 mg/kg ip) for 7d in Sprague Dawley (SD) rats。 The expression of ionized calciumbinding adaptor molecule 1 (Iba-1) was detected by immunohistochemistry in the rat dentate gyrus and cortex。 Neuronal pathological changes were observed by optical microscopy and the hematoxylin eosin staining techniques in the same areas。 Result: Compared with the control group, LPS induced microglial activation in the rat dentate gyrus and cortex。 Iba-1-positive cells were increased in LPS-treated rat。 Microglia became hypertrophic with larger and round cell bodies, and the processes of activated microglia became shorter and thicker。 LPS also induced deformation and deep staining of partial neuron。 Conclusion: LPS induce reactive microglial activation, which may be involved in neuronal damage in the rat dentate gyrus and cortex。源Q于W优H尔J论K文M网WwW.youeRw.com 原文+QQ75201.,8766

Keyword: lipopolysaccharide; brain injury; microglia; neuron

目录

摘要 2

Abstract 2

1。实验材料 5

1。1实验动物 5

1。2化学药品与试剂 5

1。3化学溶液 5

1。4实验器材 6

2。实验方法 6

2。1 动物模型建立 6

2。2 脑组织获取 7

2。3 石蜡包埋 7

2。4 载玻片前处理 7

2。5 石蜡切片 8

2。6 免疫组织化学染色 8

2。7 HE染色 9

2。8 显微镜操作 10

2。9 统计学处理 10

3。实验结果 11

3。1脂多糖刺激大鼠海马DG区小胶质细胞激活 11

3。2脂多糖刺激大鼠皮层小胶质细胞激活 12

3。3脂多糖给药对大鼠海马和皮层神经元的影响 13

4。结果讨论 13

参考文献 15

致谢 17

引言

根据目前研究表明，脑损伤与中枢神经退行性疾病有关[1]。中枢神经系统炎症反应在中枢神经退行性疾病阿尔茨海默病（Alzheimer’s disease, AD）、帕金森病（Parkinson’s disease, PD）、痴呆（frontotemporal dementia）以及肌萎缩性脊髓侧索硬化症（amyotrophic lateral sclerosis，ALS）的发病机制和进展中是一个重要的特征。