摘  要研究目的：利用四溴联苯醚(2,2',4,4'-tetrabromodiphenyl ether, BDE-47) 建立小鼠肝脏损伤模型， 研究曲克芦丁(Troxerutin)对BDE-47诱导的小鼠肝脏炎症损伤的影响及分子机制。方法：24只小鼠，随机等量分成4组：Control组、BDE-47组(150mg/kg/d)、BDE-47+Troxerutin组(150mg/kg/d)和Troxerutin 组(150mg/kg/d)，连续喂养12周后处死,检测小鼠的体重、血清中ALT活力、ROS含量、NF-κB、F4/80和IL-1β表达量。结果：曲克芦丁改善BDE-47处理的小鼠血清中ALT的活性，能降低BDE-47处理的小鼠肝脏中ROS水平，能抑制BDE-47诱导的小鼠肝脏细胞NF-κB转核。曲克芦丁还降低BDE-47处理的小鼠肝脏中炎症相关蛋白F4/80、IL-1β的表达。结论：曲克芦丁抑制BDE-47诱导的小鼠肝脏炎症反应，其机制可能为曲克芦丁能够抑制BDE-47处理的小鼠肝脏组织中的ROS的浓度，进一步下调抑制细胞因子NF-κB的活性，从而减弱炎症相关因子F4/80、IL-1β的表达，保护了肝脏，从而为曲克芦丁的临床应用提供依据。70725

该论文有图2幅，表2个，参考文献27篇。

毕业论文关键词：曲克芦丁  2,2',4,4'-四溴联苯醚  炎症反应  小鼠肝脏 

Troxerutin inhibits BDE-47-induced inflammation in mouse liver 

Abstract Objective: Mouse model of liver injury induced by 2,2 ', 4,4'-tetrabromodiphenyl ether (BDE-47) was established to explore the effects of troxerutin on inflammation in mouse liver and the molecular mechanisms underlying this action . Method: 24 mice were pided into 4 groups at random: control group , BDE-47 group(150mg / kg / d), BDE-47+ Troxerutin group (150mg / kg / d) and Troxerutin group (150mg / kg / d). Mice were killed after 12 weeks of treatment, and body weight, ALT activity, ROS content and protein expressions of NF-κB, F4/80 and IL-1β were measured. Result: Troxerutin improved ALT activity in the serum of mice treated with BDE-47, reduced the level of ROS in the livers of mice treated with BDE-47, and inhibited the nuclear translocation of NF-κB in the livers of BDE-47 treated mice. Ultimately, troxerutin suppressed the expression of inflammation related protein, such as F4/80 and IL-1β in the livers of BDE-47 treated mice. Conclusion: Troxerutin exhibited protective effects against BDE-47-induced liver inflammation by reducing the level of ROS, which thereby attenuated the nuclear translocation of NF-κB, and subsequent transcription of inflammation-related genes. This study indicates that troxerutin might be a candidate for the prevention and therapy of BDE-47-induced hepatotoxicity.

Key Words:  troxerutin  2,2',4,4'-tetrabromodiphenyl ether   inflammation  mouse liver

目  录

摘  要 I

Abstract II

目  录 III

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1 绪论 1

2 材料与方法 2

2.1实验材料 2

2.2实验仪器 3

2.3 实验方法 3

3统计学方法 4

4结果 5

4.1曲克芦丁改善BDE-47处理小鼠的肝脏损伤 5

4.2曲克芦丁降低BDE-47处理小鼠肝脏中ROS水平 5

4.3曲克芦丁抑制BDE-47诱导的小鼠肝脏细胞NF-κB转核